GIEMSA for Hp for Helicobacter pylori 75 test - Special Stains

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Product for the preparation of cyto-histological samples for optical microscopy. To demonstrate Helicobacter pylori bacterium on gastric biopsy sections. The qualitative and quantitative composition of staining mixture and the careful differentiation allow identifying bacteria on tidy ground.

METHOD:

1) Deparaffinise sections and bring to distilled water.
2) Preparation of buffer solution. Take out from bottle B 5 ml of solution and diluting 1:10: Utilize obtained solution for the preparation of Giemsa working-solution.
3) Preparation of Giemsa working-solution. Take out 10 ml of reagent A and reach the volume of 40 ml with buffer solution previously prepared.
4) Put the solution in a Coplin Jar and immerse sections for 30 minutes.
5) Drain the slide without washing and put the section for 15 seconds in reagent C.
6) Repeat step 5 with reagent D and E.
7) Clear in xylene and mount.