MASSON TRICHROME with aniline blue 100 test - Special Stains

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Product for the preparation of cyto-histological samples for optical microscopy. Recommended method for connective tissue. It demonstrates gametes, nuclei, neurofibrils, neuroglia, collagen, keratin, intracellular fibrils, and negative image of Golgi apparatus.

Method:

1) Bring section to distilled water.
2) Put on the section 6 drops of reagent A and add 6 drops of reagent B: leave to act 10 minutes.
3) Without washing, drain the slide and put on the section 10 drops of reagent C: leave to act 4 minutes.
4) Wash quickly (3-4 seconds) in distilled water and put on the section 10 drops of the reagent D: leave to act 4 minutes.
5) Wash in distilled water and put on the section 10 drops of the reagent E: leave to act 10 minutes.
6) Without washing, drain the slide and put on the section 10 drops of reagent F: leave to act 5 minutes.
7) Wash in distilled water and dehydrate rapidly through ascending alcohols, stop 1 minute in the last absolute ethanol. Clear in xylene and mount.