Perls 100 test - Special Stains

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Product for the preparation of cyto-histological samples for optical microscopy. To show reactive ferric iron in tissue sections (method 1) and for haematology and cytology (method 2).

Method:

1. METHOD FOR HISTOLOGICAL SECTIONS

1) Bring section to distilled water 2) Put on the section 10 drops of reagent A and 10 drops of reagent B: leave to act 20 minutes. 3) Wash well in distilled water. 4) Put on the section 10 drops of reagent C: leave to act 5 minutes. 5) Wash in distilled water 6) Dehydrate in ascending alcohols, clear in xylene and mount. 2.

METHOD FOR HAEMATOLOGY AND CYTOLOGY

1) Fix air-dried smears in methanol for 3 minutes. Then air dry again. 2) Put on the section 10 drops of reagent A and 10 drops of reagent B: leave to act 20 minutes. 3) Wash well in distilled water. 4) Cover the smear with reagent C (10-12 drops): leave to act 5 minutes. 5) Wash in distilled water and air dry.