Periodic Acid Schiff 100 Test Hotchkiss – Mc Manus - Special Stains

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Product for the preparation of cyto-histological samples for optical microscopy. To demonstrate normal and pathologic tissue components characterized by adjacent glycolic or aminohydroxylic groups for histological sections and for Haematology and cytology.

Method:

METHOD FOR HISTOLOGICAL SECTIONS:

1) Bring section to distilled water. 2) Put on the section 10 drops of reagent A: leave to act 10 minutes. 3) Wash in distilled water. 4) Put on the section 10 drops of reagent B: leave to act 20 minutes. 5) Wash in distilled water. 6) Put on the section 10 drops of reagent C: leave to act 2 minutes. 7) Drain the slide and without washing add 10 drops of reagent D: leave to act 2 minutes. Rinse in distilled water. 8) Put on the section 10 drops of reagent E: 3 minutes. 9) Wash in running tap water for 5 minutes. 10)Dehydrate through ascending alcohols. Clear in xylene and mount.

METHOD FOR HEMATOLOGY AND CYTOLOGY:

1) Bring section to distilled water. 2) Put on the smear 10 drops of reagent A: leave to act 10 minutes. 3) Wash in distilled water. 4) Put on the smear 10 drops of reagent B: leave to act 15 minutes. 5) Wash in distilled water. 6) Put on the smear 10 drops of reagent C: leave to act 2 minutes. 7) Drain the slide and without washing add 10 drops of reagent D: leave to act 2 minutes. Rinse in distilled water 8) Put on the smear 10 drops of reagent E: 3 minutes. 9) Wash in running tap water for 5 minutes. 10)Dehydrate through ascending alcohols. Clear in xylene mount.