PERLS VAN GIESON 100 test - Special Stains

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Product for the preparation of cyto-histological samples for optical microscopy. To show ferric iron, collagen and connective tissue in sections.

Method:

1) Bring section to distilled water.
2) Put on the section 10 drops of reagent A and 10 drops of reagent B: leave to act 20 minutes.
3) Wash well in distilled water.
4) Put on the section 10 drops of reagent C: leave to act 10 minutes.
5) Wash in distilled water.
6) Dehydrate quickly through ascending alcohols, stop for 1 minute at the last absolute ethanol. Clear in xylene and mount.