Van Gieson trichrome 100 test - Special Stains

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Product for the preparation of cyto-histological samples for optical microscopy. Recommended method for connective tissue, especially to differentiate collagen fibres from connective tissue.

Method:

1) Bring section to distilled water.
2) Put on the section 5 drops of reagent A and add 5 drops of reagent B: leave to act 10 minutes.
3) Blue in running tap water for 10 minutes.
4) Put on the section 10 drops of reagent C: leave to act 10 minutes.
5) Wash rapidly (2-3 seconds) in distilled water and dehydrate quickly through ascending alcohols, stop 1 minute in the last absolute ethanol, clear in xylene and mount.